Cytospin technique is a method of performing thin-layer preparation from fluids through a cytocentrifugation process.
During the procedure, the material is centrifuged and the cells are deposited onto a vertical microscope slide. This procedure permits a cell separation from the fluid medium.
Samples are centrifuged at 600–1,500 rpm, in special plastic sample chambers (Fig. 1) composed by a filter card, a glass slide and a filling port (Funnel). These chambers are held in position in the centrifuge device with special stainless steel cytoclips. During the procedure the cells that are denser then the suspending fluid, are projected towards the glass slide crossing the gap formed by the filter laid between the funnel and the glass slide (Fig. 2). The suspended fluid is absorbed by the filter and the cells are sedimented within a 32 mm (Fetsch et al. 2002) area of the glass slide (Fig. 2) with an unquestionable advantage of reducing screening time. This.
